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| Aguiar,D.M.; Amude,A.M.; Santos,L.G.F.; Ribeiro,M.G.; Ueno,T.E.H.; Megid,J.; Paes,A.C.; Alfieri,A.F.; Alfieri,A.A.; Gennari,S.M.. |
| O presente estudo relata a ocorrência de co-infecção entre o vírus da cinomose canina (CDV) e Toxoplama gondii em cães com sinais neurológicos. Amostras de soro e tecido nervoso (pos-mortem) de 21 cães, suspeitos de cinomose canina foram analisadas pela Reação de Imunofluorecência indireta (RIFI) para pesquisa de anticorpos contra T. gondii e N. caninum e por RT-PCR para CDV. Dezessete (80,9%) cães foram positivos para o CDV pela RT-PCR e 8 (38,1%) foram positivos para anticorpos contra T. gondii. Sete cães (41,1%) apresentaram-se positivos para ambos agentes, caracterizando processo de co-infecção. Somente 1 (4,7%) cão foi soropositivo para N. caninum (RIFI=100), entretanto este mesmo animal foi positivo para T. gondii (RIFI=4096) e para CDV (RT-PCR). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dogs; Canine distemper; Toxoplasmosis; Neospora caninum; Co-infection. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100032 |
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| Tsutsui,V.S.; Freire,R.L.; Garcia,J.L.; Gennari,S.M.; Vieira,D.P.; Marana,E.R.M.; Prudêncio,L.B.; Navarro,I.T.. |
| The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10(4) oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pork meat; Mouse bioassay; PCR; Toxoplasma gondii. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100006 |
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| Aguiar,D.M.; Cavalcante,G.T.; Marvulo,M.F.V.; Silva,J.C.R.; Pinter,A.; Vasconcellos,S.A.; Morais,Z.M.; Labruna,M.B.; Camargo,L.M.A.; Gennari,S.M.. |
| Foram testados, pela técnica de soroaglutinação microscópica aplicada à leptospirose, 329 cães provenientes do município de Monte Negro, RO, dos quais 156 eram da área urbana e 173 da área rural. Simultaneamente foi aplicado questionário para verificar a existência de possíveis fatores de risco pela regressão logística. Abordaram-se questões referentes a idade, sexo, dieta, tipo de criação, ambiente, contato com outras espécies e hábito de caça. Foram detectadas reações com títulos >100 em 27,3%, com 90 cães positivos ao agente, das quais 23,7% eram da área (37/156) urbana e 30,6% (53/173), da área rural (P>0,05). Os sorovares predominantes, reatores com títulos mais elevados, foram Autumnalis (22%), Pyrogenes (12%), Canicola (10%) e Shermani (7,5%).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Leptospira spp.; Fatores de risco; Amazônia. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100012 |
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| Funada,M.R.; Pena,H.F.J.; Soares,R.M.; Amaku,M.; Gennari,S.M.. |
| Fecal samples from 1755 dogs and 327 cats were examined for the presence of helminths and protozoan forms. From the total samples, 486 (27.7%) dogs and 103 (31.5%) cats presented at least one parasite. The main genus of parasite in dogs were Ancylostoma (12.7%), Giardia (8.5%), Cystoisosopora (4.4%), Toxocara (2.6%), and Cryptosporidium (2.4%). The ocurrence of Ancylostoma was associated to male dogs, older than one year, while Giardia, Cryptosporidium, Cystoisospora and Toxocara were associated to dogs younger than one year (P<0.05). Among cats, the most frequent parasites were Cryptosporidium (11.3%), Giardia (8.3%), Cystoisosopora (8.3%), Toxocara (6.1%), and Ancylostoma (2.1%). Cryptosporidium and Cystoisosopora were more prevalent in cats younger... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cães; Gatos; Helmintos; Protozoários; Freqüência. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500038 |
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| Labruna,M.B.; Souza,S.L.P.; Guimarães Jr.,J.S.; Pacheco,R.C.; Pinter,A.; Gennari,S.M.. |
| During January and February, 2000, 133 dogs from 21 farms located in eight diferent counties at the northern region of Paraná, Brazil, were examined for ticks. Ticks found on dogs were collected and identified. Information about dog rearing system was also obtained. Fifty-five ticks were collected on 19 dogs (14.3%) from 11 farms (52.4%). Four species were identified: Rhipicephalus sanguineus (20 ticks on nine dogs from seven farms), Boophilus microplus (15 ticks on five dogs from five farms), Amblyomma ovale (nine ticks on six dogs from four farms) and Amblyomma aureolatum (11 ticks on three dogs from one farm). The results are discussed considering the behavior of some dogs used to stroll into the woods and pasture areas whereas other dogs remained... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cão; Rhipicephalus sanguineus; Boophilus microplus; Amblyomma aureolatum; Amblyomma ovale. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500007 |
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| Horta,M.C.; Pinter,A.; Cortez,A.; Soares,R.M.; Gennari,S.M.; Schumaker,T.T.S.; Labruna,M.B.. |
| Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene, and eight fleas from Pedreira (80%) and four from Mogi das Cruzes (28%) were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rickettsia felis; Ctenocephalides felis felis; Dog; 190-kDa gene; Brazil. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300008 |
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| Freire,R.L.; Navarro,I.T.; Bracarense,A.P.F.R.L.; Gennari,S.M.. |
| Immunity to Toxoplasma gondii was studied in pigs, after vaccination with T. gondii antigens incorporated into immunostimulating complexes. Nine pigs (group 1 - G1) were inoculated subcutaneously with T. gondii iscoms (LIV-5 sample) and three doses were given at 21 and 13 day-intervals. The results were compared in other three groups of nine pigs each: animals in group 2 (G2) were immunized with the LIV-5 antigens without Quil A, animals in group 3 (G3) were inoculated with tachyzoites of RH T. gondii isolate, and animals in group 4 (G4) received no vaccination. Four animals were neither vaccinated nor challenged with T. gondii (group 5 - G5). Thirty days after vaccination, pigs were challenged orally with 5´10(4) oocysts at AS-28 T. gondii isolate.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Pig; Vaccine; Iscom. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000400002 |
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